Journal: Nature Communications
Article Title: TBC1D1 functions as a negative regulator of satellite cells for muscle regeneration
doi: 10.1038/s41467-025-65141-z
Figure Lengend Snippet: a , b Proliferative activities of shNC and TBC1D1-KD C2C12 myoblasts assayed with EdU (red) labeling. Nuclei were stained with DAPI (blue). a Representative images. Bars indicate 100 µm in length. b Quantitative results. n = 8 biological replicates. c – e Proliferative activities of Flag-TBC1D1-overexpressing L6 myoblasts assayed with EdU (red) labeling. Nuclei were stained with DAPI (blue). c Protein expression of Flag-TBC1D1 in L6 myoblasts. d Representative images. Bars indicate 100 µm in length. e Quantitative results. n = 9 biological replicates. f – h Proliferation of MuSCs attached to the myofibers isolated from WT and TBC1D1-KO mice (2-month-old) and in vitro cultured for 42 h. MuSCs were stained with PAX7 (green), Ki67 (red) and DAPI (blue). f Representative images. Bars indicate 50 µm in length. g , h Quantitative results. n = 63 (WT) and 64 (TBC1D1-KO). i TBC1D1 protein level in primary myoblasts isolated from 3-week-old WT and TBC1D1-KO mice. j , k Proliferation of primary myoblasts isolated from WT and TBC1D1-KO mice (4-week-old) and in vitro cultured for 2 days. Myoblasts were stained with PAX7 (red), EdU (green) and DAPI (blue). j Representative images. Bars indicate 50 µm in length. k Quantitative results. n = 3. Data are presented as mean values +/− SEM. Numbers on the graphs represent p values. Statistical analyses were carried out via one-way ANOVA for ( b ), and two-sided t-test for ( e ), ( g ), ( h ), and ( k ). Source data are provided as a Source data file.
Article Snippet: Rat L6 myoblasts were obtained from Dr. Amira Klip (University of Toronto, Toronto, Canada).
Techniques: Labeling, Staining, Expressing, Isolation, In Vitro, Cell Culture
